Bacterial species level identification and strain level differentiation using repetitive extragenic palindromic based amplicon sequencing with Oxford Nanopore Technology
August 27, 2018
This talk at Oxford Nanopore Technologies London Calling 2018 by Lukasz Krych from University of Copenhagen, discusses a new method for fast and cost-effective bacterial species level identification and strain level differentiation using Repetitive Extragenic Palindromic based amplicon sequencing on MinION platform. The method utilizes an optimized version of rep-PCR followed by dual-stage rep-PCR-2, during which sample specific barcodes are incorporated. DNA enrichment together with barcoding takes less than 5 hours and ensures highly repetitive and evenly distributed reads per sample. Watch this great talk here.